rabbit anti- sirtuin1  (Santa Cruz Biotechnology)

Journal: Asian Journal of Andrology

Article Title: The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes modelvia mitochondrial protection

doi: 10.4103/aja.aja_69_20

Figure Lengend Snippet: IR-61 increased Nrf2 activation and restored SIRT1 and SIRT3 expression in cavernous tissue. (a) Representative images of SIRT1 costaining with α-SMA. Scale bar = 100 μm. (b) Representative images of SIRT3 co-staining with α-SMA and quantitative analysis of the fluorescence intensity of SIRT3. Scale bar = 100 μm ( n = 4 per group). (c) Representative images of Western blot analysis of SIRT1 and SIRT3. (d) Quantitative analysis of the SIRT1 fluorescence intensity ( n = 4 per group). (e) Relative SIRT1 and SIRT3 protein levels in the cavernous tissue ( n = 4–5 each group). (f) Western blot analysis of Nrf2 and HO-1 in the cavernous tissue. (g) Relative protein levels of Nrf2 and HO-1 in the corpus cavernosum ( n = 4 per group). * P < 0.05, the indicated group compared with the control group; ** P < 0.01, the indicated group compared with the control group; *** P < 0.001, the indicated group compared with the control group; # P < 0.05, the indicated group compared with the DM group; ## P < 0.01, the indicated group compared with the DM group; ### P < 0.001, the indicated group compared with the DM group. Nrf2: nuclear factor (erythroid-derived 2)-like 2; SIRT1: sirtuin1; SIRT3: sirtuin3; HO-1: heme oxygenase; α-SMA: α-smooth muscle actin; DM: diabetes mellitus; DAPI: 4',6-diamidino-2-phenylindole.

Article Snippet: For immunohistochemical and immunofluorescence staining, the primary antibodies were rabbit anti-Desmin (1:200; Abcam, ab32362), goat anti-α-SMA (1:200; Abcam, ab21027), mouse anti-Calponin (1:200; Proteintech, 13938-1-AP, Rosemont, IL, USA), mouse anti-SM22α (1:100; Proteintech, 60213-1-Ig), rabbit anti-OPN (1:200; Abcam, ab8448), rabbit anti-Ras homolog gene family, member A (RhoA; 1:100; Proteintech, 10749-1-AP), rabbit anti-Rho-associated, coiled-coil containing protein kinase 1 (ROCK1; 1:100; Proteintech, 21850-1-AP), mouse anti-vimentin (1:200; Abcam, ab20346), rabbit anti-cleaved-caspase-3 (1:100; Abcam, ab13847), rabbit anti-sirtuin1 (SIRT1; 1:200; Proteintech, 13161-1-AP), rabbit anti-sirtuin3 (SIRT3; 1:200; Proteintech, 10099-1-AP), goat anti-NLRP3 (1:200; Abcam, ab4207), rabbit anti-caspase-1 (1:100; Abcam, ab179515), and mouse anti-apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC; 1:100; Santa Cruz Biotechnology, sc-514414, Santa Cruz, CA, USA).

Techniques: Activation Assay, Expressing, Staining, Fluorescence, Western Blot, Control, Derivative Assay